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polyclonal rabbit antibody against sec61β  (Lampire Biological)

 
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    Structured Review

    Lampire Biological polyclonal rabbit antibody against sec61β
    A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native <t>Sec61β</t> as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.
    Polyclonal Rabbit Antibody Against Sec61β, supplied by Lampire Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit antibody against sec61β/product/Lampire Biological
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit antibody against sec61β - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Formation of stacked ER cisternae by low affinity protein interactions"

    Article Title: Formation of stacked ER cisternae by low affinity protein interactions

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.200306020

    A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native Sec61β as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.
    Figure Legend Snippet: A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native Sec61β as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.

    Techniques Used: Mutagenesis, Western Blot, Expressing, Construct, Control, Transfection, Fluorescence



    Similar Products

    polyclonal rabbit antibody against sec61β  (Lampire Biological)
    90
    Lampire Biological polyclonal rabbit antibody against sec61β
    A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native <t>Sec61β</t> as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.
    Polyclonal Rabbit Antibody Against Sec61β, supplied by Lampire Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit antibody against sec61β/product/Lampire Biological
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit antibody against sec61β - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native Sec61β as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.

    Journal: The Journal of Cell Biology

    Article Title: Formation of stacked ER cisternae by low affinity protein interactions

    doi: 10.1083/jcb.200306020

    Figure Lengend Snippet: A point mutation that converts GFP to mGFP inhibits formation of OSER structures. (a) Immunoblot analysis of cells expressing unmutated or mGFP-Sec61γ lysates of equal amounts of cells untransfected (no DNA) or expressing either construct were loaded on the same gel and probed with anti-GFP or an antibody against native Sec61β as a control for equal loading. (b–d) Representative confocal micrographs of transiently transfected COS-7 cells expressing (b) mYFP-Sec61β, (c) mGFP-Sec61γ, and (d) C1(1-29)P450-mGFP reveal the absence of whorls, short karmellae, and loop structures. Other amorphous, often perinuclear, structures that are generally of much lower fluorescence intensity than cells expressing the unmutated GFP counterparts are visible. Bar, 5 μm.

    Article Snippet: The polyclonal rabbit antibody against Sec61β was prepared (Lampire Biological Laboratories) against the synthetic peptide PGPTPSGTNC (residues 2–10 plus a cysteine) of canine Sec61β conjugated to keyhole limpet hemocyanin using standard protocols.

    Techniques: Mutagenesis, Western Blot, Expressing, Construct, Control, Transfection, Fluorescence